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The concentration of the target element present in the sample can be determined by comparing the measured intensity of the elemental spectral line with that of a working standard curve, obtained by performing the same measurements on standard samples.
In the case of solid dosage forms, since the samples are analyzed as they are and not diluted prior to analysis, the standards are in fact solid dosage forms of similar formulation as the target (unknown) samples. Typically, a set of standards will be prepared in‐house, and will have concentrations varying between 80% and 120% of the nominal target compound concentration. To ensure reliability of the calibration procedure, several standard samples will be analyzed for each concentration.
Furthermore, several sites on each sample will be sampled with several laser pulses, ensuring a representative average value is obtained. Typically, the working standard curve will yield the concentration by weight of the target compound (e.g. drug, lubricant, etc.) corresponding to a given intensity of the associated specific elemental line.
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